Extended Data Fig. 6: The effect of NΔ24 and NΔ65 NTE truncations on the Pfr → Pr thermal reversion rate of full-length Arabidopsis PhyA. | Nature Plants

Extended Data Fig. 6: The effect of NΔ24 and NΔ65 NTE truncations on the Pfr → Pr thermal reversion rate of full-length Arabidopsis PhyA.

From: The structure of Arabidopsis phytochrome A reveals topological and functional diversification among the plant photoreceptor isoforms

Extended Data Fig. 6

a, Domain organization of full-length (FL) PhyA and NTE truncations starting at residue 25 (NΔ24) and 66 (NΔ65). The length of the NTE was extended for clarity. Dashed red lines represent the interaction of PΦB with NTE residues Tyr-70 and Ile-74. b, Thermal reversion rates of FL PhyA and its PSM bearing the NTE truncations NΔ24 and NΔ65. Data points and fit lines representative of three technical replicates are shown (see Extended Data Table 2 for statistical analyses). Also included are Pfr→Pr thermal reversion data for PSM fragments of Arabidopsis PhyA with comparable NTE truncations, remeasured here for completeness, see also Burgie et al.6. Because the reaction rates differ by orders of magnitude, rates in two time scales are shown: left panel, 120 min; right panel, 1200 min. SDS-PAGE gels and absorption and Pr-Pfr difference spectra for the preparations are shown in Supplementary Figs. 1 and 2.

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