Extended Data Fig. 7: ADA2b, CRY1 and CRY2 are involved in DNA damage repair in blue light.
a, b, Plant growth assays (a) and relative fresh weight measurements (b) showing enhanced sensitivity of cry1 cry2 and ada2b mutants to Zeocin under blue light. Five-day-old blue light (30 μmol/m2/s)-grown seedlings were transferred onto MS medium supplemented with Zeocin (75 μg/mL) and then grown in blue light for 21 d before they were photographed, and their relative plant fresh weights were analyzed. Relative plant weight was calculated by comparing with untreated WT and mutants, respectively. Data are presented as mean ± SD (n = 4). ****P < 0.0001 (One-way ANOVA, Tukey’s multiple comparisons test). c-f, Plant growth assays (c, e) and relative fresh weight measurements (d, f) showing that cry1 cry2 mutant and WT display similar sensitivity to MMS (c, d) and Zeocin (e, f) under red light. Five-day-old red light (30 μmol/m2/s)-grown seedlings were transferred onto MS medium supplemented with MMS (100 ppm) (d) or Zeocin (75 μg/mL) (f) and then grown in blue light for 14 d before they were photographed, and their relative plant fresh weights were analyzed. Plants were randomly collected into a pool of 15 individuals for plant fresh weight measurement (b, d, f). Relative plant weight was calculated by comparing with untreated WT and mutants, respectively. Data are presented as mean ± SD (n = 6)(d, f). ns, no statistical significance (t test).
