Extended Data Fig. 8: CRY1 and CRY2 are co-localized with γ-H2AX during DNA damage under blue light, but not in red light. | Nature Plants

Extended Data Fig. 8: CRY1 and CRY2 are co-localized with γ-H2AX during DNA damage under blue light, but not in red light.

From: Photoexcited cryptochromes interact with ADA2b and SMC5 to promote the repair of DNA double-strand breaks in Arabidopsis

Extended Data Fig. 8

a, Another duplicate of immunofluorescence staining assays related to Fig. 3a,b showing that the endogenous CRY1 and GFP-CRY2 are co-localized with γ-H2AX under blue light upon MMS treatment. b, Immunofluorescence staining assays showing that the endogenous CRY1 and GFP-CRY2 are not co-localized with γ-H2AX under red light upon MMS treatment. Etiolated WT or GFP-CRY2-OX/cry1 cry2 seedlings were incubated in liquid half MS with or without MMS (125 ppm) for 30 h, and then exposed to blue light (30 μmol/m2/s) (a) or red light (30 μmol/m2/s) (b) for 15 min before they were fixed. The nuclei isolated from these seedlings were immunostained with anti-CCT1 and/or -γ-H2AX antibodies. The DAPI, γ-H2AX (Alexa Fluor 555), and CRY1 (Alexa Fluor 594) or GFP-CRY2 signals were detected by confocal microscopy. Scale bars, 5 μm.

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