Extended Data Fig. 2: CRY1 and CRY2 directly interact with ADA2a in vitro. | Nature Plants

Extended Data Fig. 2: CRY1 and CRY2 directly interact with ADA2a in vitro.

From: Photoexcited cryptochromes interact with ADA2b and SMC5 to promote the repair of DNA double-strand breaks in Arabidopsis

Extended Data Fig. 2

a, b, Pull-down assays showing the interactions of CRY1 (a) and CRY2 (b) with ADA2a. GST-ADA2a served as bait. His-TF, His-TF-CRY1, His-TF-CNT1, His-TF-CRY2, and His-TF-CNT2 served as preys. c, d, Pull-down assays showing the interactions of CRY1 (c) and CRY2 (d) with ADA2a, ADA2b, and SMC5. GST-ADA2a, GST-ADA2b, GST-SMC5 and GST served as baits, His-TF-CRY1 and His-TF-CRY2 served as preys. Input images show CBB staining. Prey proteins pulled down by bait were detected with anti-His antibody.

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