Extended Data Fig. 2: DPA4 interacts with GIF1/2/3 in vitro and in vivo.
From: The SOD7/DPA4–GIF1 module coordinates organ growth and iron uptake in Arabidopsis
a, DPA4 interacts with GIF12/3 in pull-down assays. MBP–DPA4 was pulled down by GST–GIF1/2/3 immobilized on GST beads and analysed by immunoblotting with an anti-GST or anti-MBP antibody. b, The interaction between DPA4 and GIF1/2/3 was detected by split luciferase complementation assays. N. benthamiana leaves were co-infiltrated with the Agrobacterium GV3101 containing different plasmids combinations for 48 h and then images were determined by a CCD camera. The pseudocolor scale bar was used to indicate the range of luminescence intensity. All experiments were repeated independently twice with similar results.
