Extended Data Fig. 5: Pre-experimental setup II: Fv/Fm, absorption, and morphology.

(a) Fv/Fm values (blue gradient) and absorption values (orange, green, grey color gradient, Colors indicate measured wavelength: orange gradients = Absorption measured at λ 480 nm, green gradients = Absorption measured at λ 680 nm, grey gradients = Absorption measured at λ 750 nm) of the first pre-experimental setup (n1(I)) with temperature settings ranging from 12.7–34 °C. (b) Fv/Fm and absorption values of second pre- experimental setup (n1(II) and n2(II)) and averaged values (n1-2 Av.) with new temperature settings ranging from 8.6 -29.0 °C. (c) Photographs of the pre-experimental setups n1(I) with temperature conditions ranging from 12.7–34 °C and n2(II) with temperature conditions ranging from 8.6 to 29.0 °C after incubation on the table for 216 h (n1(I)) or 191 h (n2(II)) respectively. The photograph of pre-experiment n2(I) is not shown. (d) Differential interference contrast (DIC) micrographs of SAG 12.97 cells (pre-experimental setup n1(II)) under most extreme environmental conditions (four corners: samples 1, 6, 37, and 42) as well as under high irradiance 527.8 µmol photons m^-2 s^-1 at 20.5 °C; for each well, at least 10 micrographs were taken, all showing similar phenotypes of the cells.