Extended Data Fig. 6: Main-experimental setup (n1,2,3 (III)): Morphology and growth.

(a) Photographs of the main experimental setups n1, n2, and n3 (III) with temperature conditions ranging from 8.6 to 29.0 °C after incubation on the table for 65 h. (b) Fm measurements (maximal fluorescence) using IMAGING-PAM in various table conditions, legend on the right is a false color gradient indicating fluorescence intensity. (c) Differential interference contrast (DIC) micrographs of SAG12.97 cells grown on C-Medium (growth conditions see methods: growth conditions prior to exposure to environmental conditions); at least 10 micrographs were taken, all showing similar phenotypes of the cells. (d) Differential interference contrast (DIC) micrographs of SAG12.97 under most extreme environmental conditions (four corners: samples 1, 6, 37, and 42) as well as along an irradiance gradient at 21 °C (samples 19–24) and a temperature gradient at 130 µmol photons m^-2 s^-1 (samples 3, 9, 15, 21, 27, 33, and 39); for each well, at least 10 micrographs were taken, all showing similar phenotypes of the cells.