Extended Data Fig. 3: Silencing of LoVIL1 affects bulb growth transition and flower development.
From: Epigenetic silencing of callose synthase by VIL1 promotes bud-growth transition in lily bulbs
a, Phenotypes of TRV2-LoVIL1 silenced plants after the sprouting stage (cold storage at 4 °C for 8 weeks and then 12 °C for 2 weeks). Scale bar, 1 cm. b, Detecting the expression of virus COAT PROTEIN (CP) in SAMs of TRV2-LoVIL1 bulbs. FP (F-BOX FAMILY PROTEIN) served as the positive control. Noninfected plants were used as negative control (NC). c, Expression analysis of LoVIL1 in TRV2-LoVIL1 silenced plants using RT-qPCR. FP (F-BOX FAMILY PROTEIN) served as the internal reference gene. Data are presented as mean ± s.d. of three biological repeats (two-sided Student’s t-test). d,e, Short plants (d) with aborted or fewer flowers (e) were caused by silencing of LoVIL1. Images were captured 6 weeks after planting. Scale bars, 1 cm. f-i, Statistic analysis of flower number (f; n = 12 plants), flowering ratio (g; n = 12 plants), plant height (h; n = 14 plants), and leaf number (i; n = 10 plants) in LoVIL1-silenced plants 6 weeks after planting. TRV2 lines were used as controls. Data analysis in panels c, f-i employed a two-sided Student t-test. Data in panels f, h and i represent mean ± s.d. of the individual plants, while data in panel g are presented as mean ± s.d. of three biological replicates. M: DNA ladder marker.
