Extended Data Fig. 3: Repetitive sgRNA genes do not contribute to T-DNA concatenation.
a, Schematic representation of the sgRNA gene deletion from the No RT vector. b, DNA-qPCR of ALS in Col-0 (untransformed) and in T1 plants transformed with the No RT plasmid and the No RT plasmid with a sgRNA gene deleted. Each dot represents an individual plant (n = 21 for Col-0, n = 26 individual T1 plants for No RT and No RT, sgRNA gene deletions). Horizontal bars indicate the median. PCol-0 = 0.00000002, ns = not significantly different (Kruskal-Wallis ANOVA followed by Dunn’s test). c. Schematic representation of the sgRNA gene deletion from the ONSEN RT vector. d. DNA-qPCR of ALS in Col-0 (untransformed) and in T1 plants transformed with the ONSEN RT plasmid and the ONSEN RT plasmid with both sgRNA genes deleted. Each dot represents an individual plant (n = 22). Horizontal bars indicate the median. PCol-0 = 0.00000004, ns = not significantly different (Kruskal-Wallis ANOVA followed by Dunn’s test). e. DNA-qPCR of ALS in tobacco plants transformed by biolistic bombardment with particles coated with No RT or ONSEN RT plasmid DNA. Each dot represents an individual plant. Horizontal bars indicate the median. ns = not significantly different (two-tailed Mann-Whitney U test). ns, P > 0.05; ****, P < 0.0001.
