Extended Data Fig. 8: Affinity purification-mass spectrometry analysis of SG proteome. | Nature Plants

Extended Data Fig. 8: Affinity purification-mass spectrometry analysis of SG proteome.

From: Phenolic acid-induced phase separation and translation inhibition mediate plant interspecific competition

Extended Data Fig. 8: Affinity purification-mass spectrometry analysis of SG proteome.The alternative text for this image may have been generated using AI.

Related to Fig. 5. a, Illustration of the procedures for the separation of SG and supernatant fractions. b, Venn diagram showing the overlap between wild-type (WT) SG enriched components and components that showed significantly different SG/supernatant partition patterns comparing WT and rbp47abcc’ (Differentially enriched WT vs rbp47abcc’). c, Venn diagram showing the overlap between the 245 proteins derived from (b) and rbp47abcc’ SG enriched or depleted components. d-f, Heatmaps of log2 fold changes of the indicated sets of proteins based on the Venn diagram from (c). FC, fold change. g-j, Representative fluorescence microscopy images, the related intensity profiles and Pearson correlation coefficient (PCC) of Arabidopsis protoplasts co-expressing YFP-RBP47B and mCherry tagged RPL5A, RPL23aA, RPS20B, or RPS21B after W5 solution (control) or 1 mM SA treatment for 2 h. Scale bars, 20 µm. Data are representative of three independent experiments. The white arrows indicate the area of related intensity profiles. n = 8 for PCC quantification (mean ± s. e. m was shown).

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