Extended Data Fig. 2: SA induces foci formation of Arabidopsis SG marker proteins.

Related to Fig. 1. a, Representative images of Arabidopsis protoplasts expressing RFP-UBP1B after dH₂O (control) or 1 mM SA treatment for 3 h. Scale bars, 10 µm. Data are representative of three independent experiments. b, Roots of seven-day-old WT, rbp47b, and 35S:GFP-RBP47B plants were collected for protein extraction. Proteins were detected by RBP47B native antibody. Black arrows, target bands. *, nonspecific bands. Data are representative of three independent experiments. c. Representative immunofluorescence images of 35S:GFP-RBP47B roots (c), and WT, 35S:GFP-RBP47B, and rbp47abcc’ roots (d) using RBP47B antibody after 1 mM SA treatment for 2 h. Scale bars, 10 µm. Data are representative of three independent experiments. e, Representative images of poly(A)⁺ RNA in the roots of WT, acd6, cpr5, and snc1 seedlings. Scale bars, 10 μm. Data are representative of three independent experiments. f, Representative images of poly(A)⁺ RNA in the roots of WT treated with 1 mM SA for 5, 10, 12.5, 15, 20, 30, 60, or 120 min. Scale bars, 10 μm. Data are representative of three independent experiments. g, Representative images of poly(A)⁺ RNA in the roots of WT treated with 0.1, 0.2, 0.5, or 1 mM SA for 2 h. Scale bars, 10 μm. Data are representative of three independent experiments. h, Representative images of poly(A)⁺ RNA in the roots of WT grown on 1/2 MS plate with no SA (control) or 0.03 mM SA for 4 days. Scale bars, 10 μm. Data are representative of three independent experiments. i, Representative images of poly(A)⁺ RNA in the roots of WT pre-treated with 1 mM SA for 2 h followed by removal from SA for 0, 5, 10, 15, 20, or 30 min. Scale bars, 10 μm. Data are representative of three independent experiments. j, Representative images of poly(A)⁺ RNA in the roots of NahG seedlings with control (DMSO), 1 mM 4-HBA, protocatechuic acid, or caffeic acid treatment. Scale bars, 10 μm. Data are representative of three independent experiments.