Extended Data Fig. 4: Flowcharts for cryo-EM data processing of ABCG25WT in the apo- or ABA-bound state.
From: Structural basis for abscisic acid efflux mediated by ABCG25 in Arabidopsis thaliana
a, A flowchart for the data processing of ABCG25WT with DDM plus CHS extraction in the apo-state. Details can be found in the ‘Image processing’ session in Methods. b, A flowchart for the data processing of ABCG25WT (DDM plus CHS extraction) in the ABA-bound state. Details can be found in the ‘Image processing’ session in Methods. c, EM densities for the six transmembrane helices, CnH, CpH and E-helix of the apo-state ABCG25WT (DDM plus CHS extraction) map. Residues with notable side chains are labeled aside.
