Extended Data Fig. 6: Positional cloning of M3 and M4 mutations.
From: Ethylene produced in carpel primordia controls CmHB40 expression to inhibit stamen development
a, M3 and M4 are not altered in CmACS7 protein sequence. b, Segregation analysis of andromonoecious phenotype in M3 and M4 F2 backcross populations. Nb, total number of plants. c, Genome-wide distribution of Δ SNP index identified in bulk DNA of CharMono and M3 F2 segregant plants. d, Δ SNP index identified in bulk DNA of CharMono and M4 F2 segregant plants. The highest Δ SNP index, arrowhead, found on chromosome 2 and chromosome 3 were confirmed to correspond to the M3 and the M4 mutations, respectively. Δ SNP index is shown according to SNP position (Mb) on each chromosome. e, Phylogenic analysis of CmEIN3 related proteins in melon and Arabidopsis. Protein sequences were aligned and used to generate the neighbour-joining phylogenetic tree with 1,000 bootstrap replicates. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test are shown next to the branches.
