Extended Data Fig. 10: Analysis of etr1 and etr2 mutants.
From: Ethylene produced in carpel primordia controls CmHB40 expression to inhibit stamen development
a, Phylogenic analysis of ethylene receptors in melon and Arabidopsis. Protein sequences were aligned and used to generate the neighbour-joining phylogenetic tree with 1,000 bootstrap replicates. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test are shown next to the branches. b, qRT–PCR analysis of CmEIN3 and CmEIL1 in leaf, stem and flower buds collected at stage 6. c–e, qRT–PCR of CmERS1, CmEIN3 and CmEIL1 in LCM carpel and stamen primordia of female, hermaphrodite and male flowers. f, Sexual morphs observed in Cmetr1P253S and Cmetr2W530* TILLING mutants. Hermaphrodite flowers of Cmetr1P253S mutants showed wide variation in the size of stamens. Whereas, Cmetr2W530* mutant did not bear any hermaphrodite flowers. Scale bar, 5 mm. g, Graph showing the proportion of hermaphrodite and female flowers in monoecious melon (Wild-type), homozygous Cmetr1P253S mutant (etr1/etr1) and heterozygous Cmetr1P253S mutant (etr1/ETR1). h, At left, an adult melon WT control plant. In the middle, an adult plant homozygous for the mutation Cmetr1P253S (etr1/etr1) and heterozygous for the mutation Cmetr2W530* (ETR2/etr2) with stunted vegetative growth and complete inhibition in the florescence. At right, an adult melon plant homozygous for Cmetr1P253S and has WT ETR2 alleles. Scale bar, 15 cm. Data in b are means ± s.d. from 6 biological replicates. P values are calculated using using one- way ANOVA with Tukey’s multiple comparisons test. Data in c to e are means ± s.d. from 3 biological LCM replicates. P values are calculated using two-tailed Student’s t-test.
