Extended Data Fig. 4: The higher expression of RNR10 driven by the RNR10IRAT261 promoter in the HJX74 background resulted in reduced root growth and NO3− uptake.
a, Morphology of mature HJX74 and HJX74/pRNR10IRAT261::RNR10-GFP plants. Scale bar, 10 cm. b, Root RNR10 transcript abundance. Transcript abundance was measured relative to HJX74 (set to 1). c, Root RNR10-GFP protein abundance. HSP82 severs as loading control. The pictures of western blots represent one of the three experiments performed independently with similar results. d, Root systems of HJX74 and HJX74/pRNR10IRAT261::RNR10-GFP plants. Scale bar, 10 cm. e, The ratio [(LN-HN)/HN] of total length of visible roots. f, The absolute value of total length of visible roots. g, The ratio [(LN-HN)/HN] of total area of visible roots. h, The absolute value of total area of visible roots. i, The ratio [(LN-HN)/HN] of root to shoot biomass ratio. j, The absolute value of the ratio of root to shoot biomass. k-n, Mature plant height (k), the number of tillers per plant (l), the number of grains per panicle (m), and grain yield per plant (n). b, e, g, i, k-n, P values were generated from two-sided Student’s t tests. f, h, j, Different letters denote significant differences (P < 0.05) from a Duncan’s multiple range test. Exact P values are listed in Supplemental Information Table 11. b, e-j, Data are mean ± s.e.m. (n = 3 biologically independent samples). k, Data are mean ± s.e.m. (n = 16 biologically independent samples). l-n, Data are mean ± s.e.m. (n = 12 biologically independent samples).
