Extended Data Fig. 5: RNR10 transcript abundance, RNR10 protein accumulation and RSA of ZH11, ZH11/pAct::RNR10-Flag and ZH11/rnr10 plants.
a, RNR10 mRNA abundance in ZH11/pAct::RNR10-Flag-1, ZH11/pAct::RNR10-Flag-2 and ZH11/rnr10 plants, relative to ZH11 (set to 1). b, Comparison of RNR10 protein abundance detected by an anti-DDDDK-tag antibody in ZH11 versus ZH11/pAct::DNR1-Flag. HSP82 severs as loading control. c, Gene structure of the RNR10 gene showing the location of the CRISPR/Cas9-generated 1-bp deletion in the ZH11/rnr10 mutant. d, Comparison of RNR10 protein abundance detected by an anti-RNR10 antibody in ZH11 versus ZH11/rnr10. e-g, The absolute values of total length of visible roots (e), total area of visible roots (f) and root to shoot biomass ratio(g). a, e-g, Different letters denote significant differences (P < 0.05) from a Duncan’s multiple range test. Exact P values are listed in Supplemental Information Table 11. Data are mean ± s.e.m. (n = 3 biologically independent samples). The experiments in b and d were repeated independently at least three times with similar results.
