Extended Data Fig. 7: RNR10 interacts with DNR1 and OSKs. | Nature Plants

Extended Data Fig. 7: RNR10 interacts with DNR1 and OSKs.

From: Improving rice nitrogen-use efficiency by modulating a novel monouniquitination machinery for optimal root plasticity response to nitrogen

Extended Data Fig. 7: RNR10 interacts with DNR1 and OSKs.

a-d, Four unique peptide sequences were identified by immunoprecipitation followed by mass spectrometry assays. e-g, Three amino acids were identified by mass spectrometric analysis. h, i, SFLC assays. cLUC-tagged RNR10 was co-transformed into tobacco leaves along with nLUC-tagged OSK1 (h) or OSK20 (i). j, k, Co-IP experiments. Flag-tagged RNR10 was co-transformed into rice protoplasts with HA-tagged OSK1 (j) or OSK20 (k). The experiments in j and k were repeated independently at least three times with similar results.

Source data

Back to article page