Extended Data Fig. 8: Characterization of gas exchange in mitochondrial nad9 mutants in comparison to the wild type and three allotopically complemented lines.
From: Targeted knockout of a conserved plant mitochondrial gene by genome editing
Measurements were performed at different light intensities and at ambient CO2 concentration (400 ppm) or at 2,000 ppm CO2, which normally fully suppresses photorespiration. However, it seems possible that the altered leaf anatomy in the nad9 deletion lines (see text and Extended Data Fig. 9) leads to strongly increased mesophyll resistance to CO2 diffusion and precludes full repression of photorespiration in the mutants. (a) Assimilation rates. (b) gH2O, stomatal conductance. (c) ci, intercellular CO2 concentration in the substomatal cavity. Mean values and standard deviation are depicted. See Source Data files for a table with individual values and a version of this figure with individual data points shown. n = 6 biologically independent plants each examined over 1 experiment for 2,000 ppm CO2, n = 5 + 3 = 8 (WT), 6 + 1 = 7 (Δnad9-01), 5 + 1 = 6 (Δnad9-13), 5 + 1 = 6 (Δnad9-29), 5 + 3 = 8 (Δnad9-c03), 5 + 3 = 8 (Δnad9-c10), 5 + 3 = 8 (Δnad9-c14) biologically independent plants each examined over 2 independent experiments for 400 ppm CO2. Plants listed after the “+” sign (from experiment #2 for 400 ppm CO2) were used both for measurements at 2,000 ppm CO2 and 400 ppm CO2.
