Extended Data Fig. 3: Split-AID10 enables robust on-target editing in human and yeast cells.

a,c, Split-AID10 exhibits sufficiently high on-target editing efficiency in human HEK293T cells (a) and yeast cells (c). Data are shown as mean values and standard deviation of three biological replicates for six target loci and the highest C-to-T editing efficiency among multiple Cs was used to represent the editing efficiency at a given target locus. b,d, Split-AID10 minimizes C-to-A/G and indel byproducts in human HEK293T cells (b) and yeast cells (d). The inlaid panel in d corresponds to a magnified view. Each dot in the violin plot represents the editing efficiency for the indicated editing product per target site, while the three lines mark quartile positions. e, Domain structures of Split-AID10^N6, Split-AID10^C6, and PIGS-AID10^N6. Note that PIGS-AID10^N6 contains a complete nCas9 and an intact deaminase catalytic center. f, None of Split-AID10^N6, Split-AID10^C6 and PIGS-AID10^N6 is catalytically active in yeast cells. Data are shown as mean values and standard deviation of three biological replicates.