Extended Data Fig. 3: Cryo-EM data processing and analysis for SOS1 2class1. | Nature Plants

Extended Data Fig. 3: Cryo-EM data processing and analysis for SOS1 2class1.

From: Structural basis for the activity regulation of Salt Overly Sensitive 1 in Arabidopsis salt tolerance

Extended Data Fig. 3

a, A flowchart of the EM data processing by CryoSPARC46. 9060 micrographs were selected after CTF estimation and motion correction from 9098 movie stacks. b, Representative results of 2D classification of Class1 after particle picking and extraction. The red arrow indicates the stable CPD of Class1. c, The overall resolution of the reconstruction for SOS1 class1 is estimated to be 2.87 Å. d, Angular distribution of the particles used for reconstruction of SOS1. e, Local resolution estimation in Å of the SOS1 class1. f, FSC curves of the refined model versus the overall 2.87 Å map that it was refined against (black); of the models refined against the first half maps versus the same maps (purple); and of the models refined against the first half maps versus the second half maps (green). The small difference between the red and green curves indicates that the refinement of the atomic coordinates did not suffer from overfitting.

Back to article page