Extended Data Fig. 7: Structure-based sequence alignment of CNBDs.
From: Structure and activation mechanism of the rice Salt Overly Sensitive 1 (SOS1) Na+/H+ antiporter
The structures for both CNBDs (OsSOS1 and HCN) have been superimposed and used to restrict sequence gaps to inter-secondary structural elements. Superior coils and arrows define extents of the secondary structural elements in OsSOS1 CNBD (top) and HCN CNBD (bottom). Two loss-of-function mutations in the b-roll structure of the CNBD, including G777D (sos1-8) and G784E (sos1-9), led to impaired transport function in Arabidopsis are highlighted in magenta. The residues critical for cAMP binding in the HCN CNBD are highlighted in cyan.
