Extended Data Fig. 1: Structure-based sequence alignments for the representative SOS1s from both dicots and monocots.
From: Structure and activation mechanism of the rice Salt Overly Sensitive 1 (SOS1) Na+/H+ antiporter
The cryo-EM structure of OsSOS1 is used to restrict sequence gaps to inter-helical segments, with the superior coils and arrows defining extents of the secondary elements. The sequences include representative members from dicots and monocots: Oryza sativa Japonica Group (XP_015619351.1), Zea mays (XP_008645741.2), Sorghum bicolor (XP_002443674.1), Aegilops tauschii (XP_020190641.1), Brachypodium distachyon (XP_003576505.1), Dendrobium officinale (XP_020699162.1), Asparagus officinalis (XP_020256864.1), Musa acuminata subsp. malaccensis (XP_018686090.1), Phoenix dactylifera (XP_008798100.1), Amborella trichopoda (XP_006849492.1), Arabidopsis thaliana (NP_178307.2), Brassica rapa (XP_009114217.1), Eutrema salsugineum (XP_006395824.1), Beta vulgaris subsp. vulgaris (XP_010680103.2), Glycine max (NP_001244939.1), Daucus carota (XP_017226136.1), Cucumis melo (XP_008466844.1), Vitis vinifera (NP_001268140.1), Juglans regia (XP_018828660.1), Eucalyptus grandis (XP_010066529.2). The highly conserved inhibitory motifs (motif-1 and motif-2) are shown in boxes. Some critical residues are highlighted as following: 1. The putative phosphorylation residues are highlighted in cyan (S1113 and S1135 in OsSOS1). 2. The mutated residues are highlighted in yellow.
