Extended Data Fig. 2: Structural determination of the OsSOS1FL.
From: Structure and activation mechanism of the rice Salt Overly Sensitive 1 (SOS1) Na+/H+ antiporter
(a) The workflow for image processing of the OsSOS1FL. 2D classification of the OsSOS1FL particles from the cryo-EM sample are shown. (b) Fourier shell correlation (FSC) curve suggests an overall resolution at 3.1 Å, as estimated using the 0.143 cut-off criterion (Left). The FSC curves for cross-validation between the model and the unmasked (Black) or masked (Pink) cryo-EM maps of OsSOS1 (Right). (c) Representative cryo-EM density map for the TM segments of TMD, helical elements of HD, CNBD, CTD and auto-inhibitory motif segments.
