Extended Data Fig. 1: Capture of regulatory factors of SE.
a, Representative phenotypes of the CRISPR/dCas9-gRNA1, -gRNA2, -gRNA3 and -gRNA4 lines compared with wildtype (WT). Scale bar, 1 cm. b, The dCas9-6Myc enrichment at gRNA3 targeting locus. The y axis shows the calibrated relative ratio of bound DNAs to input DNAs after IP, and IgG serves as the negative control. The dCas9-6Myc enrichment at a random region of AGO1 promoter was used as the reference. Values are the means ± s.d. of three biological replicates. c, The enrichment of precipitated peptides in the CRISPR/dCas9-gRNA3 line compared with the non-gRNA control from LC‒MS/MS data by volcano plot. The x axis represents the peptide number change (peptide number in the non-gRNA control minus peptide number in the CRISPR/dCas9-gRNA3) and the y axis represents the normalized P value. Peptides specific in the CRISPR/dCas9-gRNA3 line (Specific-gRNA3) are shown in cyan and peptides specific in the non-gRNA control (Specific-control) are shown in red (p < 0.05 by one-way ANOVA). The p values for PAR2, WRKY19 and MYB27 peptides are 0.004, 0.020 and 0.038, respectively. d, Mass spectrometry analysis of PAR2, WRKY19 and MYB27 peptides. e-g, Gene structures (upper panel) and expressions (lower panel) of PAR2 (e), WRKY19 (f) and MYB27 (g) in par2-1, wrky19-2 (w19-2) and myb27-1 mutants, respectively. The Inverted triangles and blue triangles represent the relative position of the T-DNA and the position of the primers used for qRT-PCR analysis, respectively. #1, #2 and #3 in (e-g) represent different individual plants in the same T-DNA insertion lines. Values are the means ± s.d. of three biological replicates. In b and e-g: **p < 0.01, two-sided Student’s t-test. The exact p values are shown in Supplementary Data 4.
