Extended Data Fig. 2: Representative phenotypes of different mutants and analysis of miRNAs formation in par2-1 and myb27-1 mutants.
a, Representative phenotypes of the par2-1 mutant as compared to WT. Photographs were taken of 3-wk-old seedlings. Scale bar, 1 cm. b, The representative leaves of par2-1, wrky19-2 and myb27-1 mutants as compared to WT and se-1. Photographs were taken for the second, third, and fourth true leaves of 3-week-old seedlings. Scale bar, 1 cm. c, Numbers of serrations per side of each leaf (n = 75) of par2-1, wrky19-2 and myb27-1 mutants as compared to WT and se-1. Values are the means ± s.d. (n = 75). d, Accumulation of miRNAs (miR157, miR162 and miR164) in the WT and par2-1 mutant by northern blotting. U6 snRNA was used as the control. e, qRT‒PCR analysis of target genes of the miRNAs in (d) in the WT and par2-1 mutant. Values are the means ± s.d. of three biological replicates. f, qRT‒PCR analysis of miRNAs (miR157, miR162 and miR164) in the WT and myb27-1 mutant. Values are the means ± s.d. of three biological replicates. g, qRT‒PCR analysis of target genes of the miRNAs in (f) in the WT and myb27-1 mutant. #1, #2 and #3 in (e-g) represent different individual plants in the same T-DNA insertion lines. Values are the means ± s.d. of three biological replicates. h, The comparison of SEAIR expressions between pSEAIR:SEAIR and pSEAIR:SEAIR plus p35S:PAR2 in N. benthamiana leaves. Values are the means ± s.d. of three biological replicates. i, The comparison of SEAIR expressions between pSEAIR:SEAIR and pSEAIR:SEAIR plus p35S:MYB27 in N. benthamiana leaves. #1 and #2 in (h-i) represent different individual plants from the same expressing group. Values are the means ± s.d. of three biological replicates. In c and e-i: *p < 0.05; **p < 0.01; ns: no significance, two-sided Student’s t-test. The exact p values are shown in Supplementary Data 4.
