Extended Data Fig. 3: Representative phenotypes of par2-1 pPAR2:PAR2-6Myc and myb27-1 pMYB27:MYB27-GFP plants. | Nature Plants

Extended Data Fig. 3: Representative phenotypes of par2-1 pPAR2:PAR2-6Myc and myb27-1 pMYB27:MYB27-GFP plants.

From: Capture of regulatory factors via CRISPR–dCas9 for mechanistic analysis of fine-tuned SERRATE expression in Arabidopsis

Extended Data Fig. 3

a, Representative phenotypes of par2-1 pPAR2:PAR2-6Myc (upper) and myb27-1 pMYB27:MYB27-GFP plants (lower). Scale bar, 1 cm. b-c, qRT-PCR analysis of SE expressions in par2-1 pPAR2:PAR2-6Myc (b) and myb27-1 pMYB27:MYB27-GFP plants (c). Values are the means ± s.d. of three biological replicates. d-e, Accumulation of miRNAs (miR157, miR162 and miR164) in par2-1 pPAR2:PAR2-6Myc (d) and myb27-1 pMYB27:MYB27-GFP plants (e). Values are the means ± s.d. of three biological replicates. f-g, qRT‒PCR analysis of target genes of the miRNAs in (d-e) in par2-1 pPAR2:PAR2-6Myc (f) and myb27-1 pMYB27:MYB27-GFP plants (g). #1, #2 and #3 in (b-g) represent different individual plants in the same transgenic lines. Values are the means ± s.d. of three biological replicates. In b-g: *p < 0.05; **p < 0.01; ns: no significance, two-sided Student’s t-test. The exact p values are shown in Supplementary Data 4.

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