Extended Data Fig. 9: CRISPR deletes the majority of SBT3.3/3.4/3.5 and SBT3.6/3.7/3.8/3.9/3.10 genomic region.
From: Subtilase-mediated biogenesis of the expanded family of SERINE RICH ENDOGENOUS PEPTIDES
(a) Schematic view of the large genomic deletion of clusters SBT3.3/3.4/3.5. The fusion protein may be transcribed, consisting of the first 331 amino acids of SBT3.5 with an out-of-frame fusion with SBT3.3 leading to an early stop. Primers F1/R1 and F2/R2 used for the genotyping and sequence (Supplementary Table 3). (b) Schematic view of the large genomic deletion of clusters SBT3.6/3.7/3.8/3.9/3.10. One fusion protein driven by the promoter SBT3.7 may be transcribed, consisting of 212 amino acid of SBT3.7, fused to 12 nonsense amino acids from SBT3.6 genomic region. Another fusion protein driven by the promoter SBT3.6 may be transcribed, consisting of 726 amino acids of SBT3.6, fused to 11 nonsense amino acids from SBT3.7 genomic region. Primers F3/R3 and F4/R4 used for the genotyping and sequence (Supplementary Table 3).
