Extended Data Fig. 7: Localization of IDR-truncated MIDD1 in root xylem vessels. | Nature Plants

Extended Data Fig. 7: Localization of IDR-truncated MIDD1 in root xylem vessels.

From: Microtubule-associated phase separation of MIDD1 tunes cell wall spacing in xylem vessels in Arabidopsis thaliana

Extended Data Fig. 7: Localization of IDR-truncated MIDD1 in root xylem vessels.

(a) Three-dimensional structure of MIDD1 predicted by AlphaFold2. Unstructured IDR is indicated at the N-terminus. (b) Microtubules labeled with EYFP-TUB6 (pIRX3:EYFP-TUB6); full-length mScarlet-i-MIDD1(pMIDD1:mScarlet-i-MIDD1) or truncated mScarlet-i-MIDD1 lacking 1–20 amino acids (MIDD1Δ20), 1–40 amino acids (MIDD1Δ40), 1–59 amino acids (MIDD1Δ59), or 1–20/40–59 amino acids (MIDD1Δ1–20/40–59) labeled with mScarlet-i expressed in protoxylem and metaxylem cells. White arrowheads indicate MIDD1 speckles. Experiments were repeated twice with similar results. Scale bar = 5 μm. (c) Density of stripe patterns (n = 12 cells for WT, 16 cell for midd1 midd2, 13 cells for midd1 midd2/pMIDD1:mScarlet-i-MIDD1, 13 cells for midd1 midd2/pMIDD1:mScarlet-i-MIDD1Δ20, 12 cells for midd1 midd2/pMIDD1:mScarlet-i-MIDD1Δ40, 12 cells for midd1 midd2/pMIDD1:mScarlet-i-MIDD1Δ59, 13 cells for midd1 midd2/pMIDD1:mScarlet-i-MIDD1Δ1-20/40-59, each cell was measured from 1 plant). Letters indicate statistically significant differences (p < 0.05) determined by one-way ANOVA, followed by Tukey’s test. Box plot indicates the median (horizontal line) and the interquartile range from 25 to 75%, and whiskers indicate SD. Images were obtained by spinning disk confocal microscopy. Scale bar = 10 μm.

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