Extended Data Fig. 6: Other epigenetic changes in semi-syn18L.
From: A designer synthetic chromosome fragment functions in moss
(a) Distribution of five histone modifications (H3K4me3, H3K9me2, H3K27me3, H3K36me3 and H3K27ac) on genes in the synthetic region. The signals shown were normalized using the BPM method (Bins Per Million mapped reads, same as TPM in RNA-seq) after deducting the input. (b) The number of peaks for histone modifications in wild-type and semi-syn18L. The left figure shows the number of histone modification peaks across the entire genome, while the right figure shows the number of histone modification peaks in the replacement region. The counts of three replicates were separately calculated. (c) Changes in H3K9me2 modification and DNA methylation levels at the remaining intergenenic region in semi-syn18L. The top panel shows designed chromosome fragment. The Xs on the top panel refer to new joints formed by deleting duplicate sequences, which correspond to the yellow delete regions below.
