Extended Data Fig. 1: The design and assembly of three 20–30 kb mid-chunks.
From: A designer synthetic chromosome fragment functions in moss
We split the 68,530 bp mega-chunk into three mid-chunk fragments and added a resistant cassette (kanamycin or hygromycin) to the centromeric end of each mid-chunk. If it is not possible to achieve the goal of replacing three 30 kb mid-chunks in the assembly, the replacement of two or one mid-chunks should be implemented. Each mid-chunk overlapped with the next one by a 1 kb overhang and 1 kb homologous arms were included at either end of the entire region.
