Extended Data Fig. 8: Analysis of H3K4me3 and H3K27me3 on TaVRN1 and TaVRN2 chromatin.
a, ChIP-qPCR examination of H3K4me3 at TaVRN1 and the internal control TaAPSM1 (clathrin-associated adaptor protein complex 1 subunit mu-1). The levels of VRN1-In1 are as described in Fig. 5c. b, ChIP-qPCR examination of H3K27me3 at TaVRN1 and the internal control TaACT. The levels of VRN1-In1 in mature embryos and spikelets are as described as Fig. 5b. c, d, ChIP-qPCR examination of H3K27me3 (c) and H3K4me3 (d) at TaVRN2a. Levels of the immunoprecipitated TaVRN-A/B/D2a fragments by anti-H3K4me3 or anti-H3K27me3 were quantified by qPCR and directly normalized to input DNA. a-d, Data are presented as mean values ± s.d. of three biological replicates. P values in (b) are calculated using one-way ANOVA to compare multiple means (transformed by square root) or a two-tailed t-test for comparing two means. P values in (c-d) are calculated using one-way ANOVA.
