Extended Data Fig. 6: Floral phenotypes in WT, grp20, and the complementation plants (ProGRP20::GRP20-YFP; grp20), and flower phenotypes of double mutants between GRP20 and floral homeotic genes or LOB domain gene.
a, Sepal and carpel defects in grp20 mutants and complementation lines. WT flowers show normal organ number and morphology. Flower defects are shown in grp20-1 including in the left panel, the white asterisk indicates a floral bud at the position of a sepal in grp20-1; a white arrow in the middle panel indicates an unfused carpel in grp20-1 and yellow arrows in the right panel indicate an extra sepal and petal, respectively. Flower defects were shown in grp20-2 including an extra sepal (indicated by an orange arrow; total 5) and a curved carpel (indicated by a white arrow) are shown in the left and middle panels, respectively; an extra sepal and extra petal (total 5 sepals and 5 petals) in the right panel are indicated by orange arrows. Normal flowers are shown in ProGRP20::GRP20-YFP;grp20 complementation line. Bar = 1 mm. The yellow letter and number at top right of each panel indicate corresponding organ and number in the flower. Se: Sepal; P: Petal. The white number indicates sepal count in a flower. b, Scanning electron microscope (SEM) images of floral phenotypes in WT (left) and grp20-1 (right). An extra stamen is indicated by an orange arrow in the left panel in grp20-1. Fused anthers and petals are indicated by the orange asterisks in the middle panel and the right panel, respectively in grp20-1. The positions of missing stamens are indicated by dashed orange arrows in the right panel in grp20-1. Bar = 20 μm. c, Petal length, width, and top angle in WT, grp20 and complementation lines. Petal length (pl), width (pw) and top angle (α) are indicated by the orange double arrowed line, pink double arrowed line, and light green angle α, respectively. Bar = 100 μm. d, Reduced pl in grp20-1: (difference of pl in WT and grp20-1)/(average pl in WT) = (0.98-0.84) mm /0.98 mm = 14%. Flower counts for petal length: WT, 15; grp20-1, 18; and ProGRP20::GRP20-YFP grp20, 18. Slightly reduced petal width in WT, grp20 and complementation plants. Flower counts for petal width: WT: 16, grp20-1: 20 and ProGRP20::GRP20-YFP; grp20: 18. Reduced top angle α in grp20-1. (Reduced α in grp20-1)/(average α in WT)= (140-83)/ 140 = 40%. Flower counts: WT: 16; grp20-1: 15; ProGRP20::GRP20-YFP;grp20: 15. two-sided Student’s t test is used for statistics; The lower bound, maxima, minima, centre and upper bound of box plots (from left to right) is shown as: For pl: WT: 1.36, 1.41, 1.29, 1.375, 1.39; grp20-1: 1.105,1.24, 1.04, 1.2, 1.225; GRP20-YFP (grp20): 1.33, 1.43, 1.28, 1.36, 1.39. For pw: WT: 0.41, 0.46, 0.39, 0.42, 0.43; grp20-1: 0.39, 0.42, 0.38, 0.41, 0.42; GRP20-YFP (grp20): 0.42, 0.46, 0.39, 0.43, 0.435. For top angle α: WT: 136, 152, 132, 83.6, 92; grp20-1: 75, 101, 72, 83.6, 92; GRP20-YFP (grp20): 134.25, 154, 132, 146, 148.75. e, Stamen phenotypes in grp20 mutants. f, Petal morphologies in grp20 mutants. g, Carpel phenotypes in grp20 mutants. Bar = 300 μm. h, Western blot analysis of GRP20-YFP in two complementation lines (C1 and C2) with the ProGRP20::GRP20-YFP transgene in grp20. A GFP antibody was used against whole cell lysate from transgenic flowers. i, Mature flowers of double mutants between grp20 and homeotic mutants ap1-1, ap2-1 and LOB domain mutant lbd7-1. The yellow arrows indicate extra stamen in grp20-1 and grp20-1 lbd7-1. The asterisks indicate chimeric organs in ap1-1, grp20-1 ap1-1, ap2-1 and grp20-1 ap2-1. The double mutants grp20-1 ap1-1, grp20-1 ap2-1 and grp20-1 lbd7-1 show more severe floral organ defects including various organ numbers compared to single mutants. Bar = 1 mm.
