Fig. 2: Splicing events in WT and grp20 flowers and leaves.
a,b, Number of genes whose transcripts had pre-mRNA splicing defects (ES, A3SS and A5SS) in grp20 flowers (a) and leaves (b). Flower specific, different transcripts found only in flowers. Flower and leaf, different transcripts found in both flower and leaf. Leaf specific, different transcripts found only in leaves. c, ΔPSI of alternative transcripts in grp20 and WT flowers, for floral homeotic genes (MADS-box genes and AP2), AP2 family genes, LOB domain genes, histone-binding genes, DNA and histone writers, and stress-responsive genes. Data are shown as the absolute value of ΔPSI (ABS (ΔPSI)) in c and d. P values for all transcripts in c and d are <0.05. The ΔPSI and P value of each affected transcript are shown in Supplementary Table 1. ES, A3 (A3SS), A5 (A5SS) and AI (ASI) at the bottom represent the splicing type in the transcripts. Type indicates the corresponding splicing defective category: ES, A3SS, A5SS and ASI. d, ΔPSI of alternative transcripts in grp20 and WT leaves, for histone and DNA modification genes, WRKY and NAC transcription factors, MADS-box genes, circadian rhythm genes, protein ubiquitinating genes and genes that respond to ABA and light. e,f, Relative levels of wild-type transcripts of skipped exons of floral homeotic genes in grp20-1 flowers (e), and WRKY family genes, histone methylation binding gene ING2 and protein ubiquitination gene RHC1A in grp20-1 leaves (f). P (AG) = 0.0033; P (STK) = 0.00018; P (AP3) = 0.0055; P (PI) = 0.0017; P (AP2) = 0.0013; P (SEP4) = 0.0081; P (AP1) = 0.00078; P (ING2) = 0.0031; P (WRKY20) = 0.0097; P (WRKY26) = 8.27 × 10−5; P (RHC1A) = 0.00301. The primers are listed in Supplementary Table 7. The data are presented as mean ± s.e.m. from three biological replicates for e and f. **P < 0.01, two-sided Student’s t-test in e and f.
