Extended Data Fig. 2: IDB can be used directly for Pi staining in plants.
From: Visualizing plant intracellular inorganic orthophosphate distribution
The germinated wild type (Nip) seeds were directly sown on +P (200 μM Pi) cultural solution for 7 days before the Pi-staining assay. Total roots were directly stained with IOSA detecting buffer (+IDB) under vacuum conditions for 20 min. The reaction buffers of H2O (without IDB: –IDB), +AM (only plus ammonium molybdate), and IDB(–AM) (IDB buffer without ammonium molybdate) were used as negative control. Scale bar, 1 cm.
