Extended Data Fig. 2: Sperm cell development and fertility is not affected in the ntl4 ntl11 double mutant.
From: EGG CELL 1 contributes to egg-cell-dependent preferential fertilization in Arabidopsis
a, NTL4 and NTL11 gene structure showing the loci of T-DNA insertion in ntl4 (nac53-1) and ntl11 (nac78-1). b, DAPI staining of wild type (n = 466) and ntl4 ntl11 (n = 396) pollen. The mean percentage of pollen containing two sperm nuclei (SN) is shown (with SD in brackets). VN, vegetative cell nucleus. c, The ntl4 ntl11 mutant has a seed set comparable to that of the wild type (Col-0). Mean quantities of seeds formed (in %) with SD in brackets are indicated at the upper right. n = 9 siliques (597 ovules) for Col-0 and 10 siliques (664 ovules) for ntl4 ntl11. Scale bars, 10 µm in b and 1 mm in c.
