Fig. 2: SPT directly interacts with SEC and SPY.
From: O-glycosylation of the transcription factor SPATULA promotes style development in Arabidopsis
a, Co-IP experiments performed in tobacco leaves showing the full-length SPT–FLAG protein co-immunoprecipitated with full-length SEC–HA (left) or SPY–HA (right) recombinant proteins. The panel shows representative results from one of three independent experiments. b, Split-luciferase assay performed in tobacco leaves showing the interaction between full-length SPT–nLuc with both SEC–cLuc (left) and SPY–cLuc (right). The panel shows representative results from one of three independent experiments. c, Y2H assay displaying SPT domains (left) sufficient for interactions with full-length SPY and SEC proteins. Positive co-transformants were selected on SD-LW media (middle) and grown on SD-LWHA, 2.5 mM 3-AT media (right) to determine strong interactions. Ø, empty vector.
