Fig. 3: Loss of SEC function in a spy mutant background affects both vegetative and reproductive development.
From: O-glycosylation of the transcription factor SPATULA promotes style development in Arabidopsis
a, SEM analysis of stage-12 gynoecia of Col-0, spy-3 and SEC RNAi spy-3 transgenic lines (two lines of a1SEC RNAi spy-3 and four lines of a2SEC RNAi spy-3) treated with either mock (top) or DEX (bottom). Scale bars, 100 μm. b, qRT-PCR analysis of SEC expression levels from inflorescences of SEC RNAi spy-3 transgenic lines (a1SECs and a2SECs) and controls, treated with either mock (white bars) or DEX (grey bars). The values shown are means ± s.d. from three biological repeats. The P values indicate two-tailed t-test results. c, Measurement of style length of the genotypes depicted in a,b. The box plots indicate the median (the line within the box), the lower and upper quartiles (the box edges), and the largest and smallest data points (the whiskers). For Col-0, spy-3 and a1SEC RNAi spy-3 transgenic lines, n = 15 for mock and DEX treatments; for the a2SEC RNAi spy-3 transgenic lines, n = 15 for mock and n = 18 for DEX treatment. Note that the strong reduction in style elongation (a,c) correlates with low levels of SEC expression (b). The P values are indicated in the graph following two-tailed Mann–Whiney tests. d, SEM micrographs showing wild-type and SEC RNAi spy-3 (a2SEC line 3) styles (see the red boxes in a) displaying defects in cell elongation. Scale bars, 50 μm. The panel shows representative results from one of three independent experiments. e, Representative images of fruits in different stages of development after mock (top) and DEX (bottom) treatments of wild-type and SEC RNAi spy-3 (a2SEC line 3) inflorescences. Scale bars, 1 cm. f, Root length measurements of seven-day-old Col-0, spy-3 and SEC RNAi spy-3 (a2SEC line 3) seedlings grown on either mock or DEX plates. Significant differences are indicated in the graph following two-tailed Student’s t-tests. The box plots indicate the median (the line within the box), the lower and upper quartiles (the box edges), and the largest and smallest data points (the whiskers). n = 17 seedlings were analysed for each genotype and each treatment. The P values indicate the results of two-tailed t-tests. g, Representative images of 13-day-old Col-0, spy-3 and SEC RNAi spy-3 (a2SEC line 3) seedlings grown on either mock or DEX plates. Scale bars, 1 cm.
