Fig. 4: The symmetry-breaking phenotype of the spt-12 mutant style is epistatic to both sec and spy mutations.
From: O-glycosylation of the transcription factor SPATULA promotes style development in Arabidopsis
a, Representative SEM micrographs of stage-12 gynoecia of single and double mutants. Scale bars, 500 μm. The experiment was performed at least three times for each genotype. b, Quantification of the frequency and severity of the bilaterally symmetric defective styles observed in the double sec spt and spy spt mutants compared with their parental lines. Phenotypes were grouped in the following four categories: radial (wild-type-like style, radially symmetric), weak (shallow cleft at the organ’s distal tip), medium (cleft running through the middle of the style) and severe (deep cleft spanning the style into the ovary region). Fifty gynoecia were analysed for each genotype. The phenotypic classes were compared with spt-12 using 4 × 2 contingency tables followed by Pearson’s χ2 test. The two-tailed P values are as follows: &, P < 0.0001; #, P < 0.00001; ^ P = 0.000019; NS, not significant P > 0.05 (sec-2 spt-12 versus spt-12, P = 0.0249; sec-5 spt-12 versus spt-12, P = 0.1007). P values <0.01 were considered statistically significant. Scale bars, 100 μm. c, Quantification of the severity of the bilateral split style (relative cleft depth) was measured as the depth of the cleft (I) divided by the style length (L). More than 60 gynoecia were analysed for each genotype. The internal dashed lines indicate the median and quartiles. Phenotypic classes were compared with spt-12 using ordinary one-way analysis of variance (ANOVA) multiple comparisons. Scale bar, 100 μm.
