Fig. 3: Gamete-sequencing derived meiotic recombination dynamics in R. breviuscula.

a, Recombination landscape of the five chromosomes in R. breviuscula achieved by computing 4,047 COs from 1,641 pollen nuclei. The black line shows the CO rate; shadow ribbons indicate one standard deviation from mean CO rates; blue dashed vertical lines indicate the start and end of confident CO rate computation (Supplementary Fig. 9); blue solid vertical lines mark the chromosomal end; magenta horizontal lines indicate the genome-wide average CO rate; green horizontal lines are the chromosome-wide average CO rate; and orange bars indicate large (>2 Mb) homozygous regions with a reduced number of markers. b, Genetic linkage map computed from COs in 81 F₀ pollen nuclei having more than 2,000 markers. Genetic length density is indicated by the coloured scale. A set of 705 markers was selected using a 500 kb sliding window through all markers defined against the reference (Methods). The terminal locations of the 35S rDNA locus on chr1 and chr2 are indicated by asterisks in a and b. c, Marey map calculated from the linkage map in b. Marey maps for each chromosome (colour lines) show genetic position as a function of physical position. d, CO number derived by counting CO events from the genetic analysis in pollen nuclei having more than 2,000 markers compared with the one extrapolated from the F₁ offspring. The box plots are comprised of minima, Q1, mean, Q3 and maxima following the definition of ggplot2 in R. N indicates biologically independent pollen nuclei and F₁ offspring individuals used for CO detection. e, Distribution of CO number for each single chromatid in gametes. Note the higher incidence of double COs on chr3. f, CoC curve in pollen nuclei (n = 1,641). Chromosomes were divided into 15 intervals, with random sampling at CO intervals, to calculate the mean CoC of each pair of intervals.