Extended Data Fig. 2: Immunolocalisation of ASY1, REC8, and ZYP1 from leptotene to pachytene of meiotic prophase I. | Nature Plants

Extended Data Fig. 2: Immunolocalisation of ASY1, REC8, and ZYP1 from leptotene to pachytene of meiotic prophase I.

From: Meiotic recombination dynamics in plants with repeat-based holocentromeres shed light on the primary drivers of crossover patterning

Extended Data Fig. 2

(a) ASY1 (green) appears as a linear signal on unpaired chromosomes. Behaviour of ASY1 was consistent in all meiocytes analysed (n = 10) among 6 independent experiments. (b) Full co-localisation of cohesin protein REC8 (green) and ZYP1 (magenta) at pachytene. Behaviour of REC8+ZYP1 was consistent in all meiocytes analysed (n = 12) among 3 independent experiments. A maximum projection is shown; chromosomes were counterstained with DAPI. Scale bars, 5 µm.

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