Extended Data Fig. 6: RLP4s are required for RAB-A5c localization.

(A,B) CLSM maximum intensity projections of lateral root epidermal meristem cells expressing YFP–RAB-A5c in wild type (A) or rlp4 rlp4-l1 (B) backgrounds. The cell wall was stained with propidium iodide. Cell plates are labelled with white arrows. Experiments were conducted 2 times independently, representative images are shown. (C) Photographs of 10d old Col-0, rlp4, rlp4-l1, and rlp4 rlp4-l1 seedings. (D) CLSM maximum intensity projections of lateral roots from seedlings shown in (C). The cell wall was stained with propidium iodide. Experiments were conducted 2 times independently, representative images are shown. (E) Violin plots of the mean maximum diameter of lateral roots from plants after 3d treatment with DMSO or Oryzalin. N for plants treated with DMSO or Ory, respectively, was 32, 35 (pRLP4::RLP4–GFP) or 22, 37 (pRLP4-L1::RLP4-L1–GFP). Difference in diameter (%) between DMSO (white) and Ory (yellow) treatments for each genotype noted above respective columns. Relative swelling in response to Ory is significantly increased compared to wild type plants (p = 0.005 and 0.028, respectively), but less so than in rlp4 rlp4-l1 plants examined in the same experiment (Fig. 4G; p < 0.05, two-way ANOVA and post-hoc Tukey test). Experiment was conducted 3 times independently, and results from one representative experiment are shown. Violin plots are defined in the Methods section. (F) Representative images of lateral roots after 3d treatment with DMSO or Oryzalin from rlp4 rlp4-l1 pRLP4::RLP4–GFP, rlp4 rlp4-l1 pRLP4-L1::RLP4-L1–GFP, pRLP4::RLP4–GFP, pRLP4-L1::RLP4-L1–GFP plants as those in quantified in Fig. 4G, S6E. Experiments were conducted 3 times independently, representative images are shown. Scale bars 5 µm (A,B) or 100 µm (D,F).