Fig. 2: RLP4s associate with the cell wall.
From: A self-regulatory cell-wall-sensing module at cell edges controls plant growth
a, Schematic depiction of truncated RLP4 variants. MLD, malectin-like domain; LRR, leucine-rich repeat; TMD, transmembrane domain; ID, intracellular domain; SP, signal peptide. b,c, CLSM maximum-intensity projections of lateral root epidermal meristem cells co-expressing pUBQ10::secRFP–RLP4ΔECD with Golgi and TGN/EE markers. The experiments were conducted at least four times independently; representative images are shown. d, Manders’s colocalization coefficients showing the fraction of RLP4s–RFP colocalizing with the membrane markers shown in b,c. Three or more CLSM stacks of lateral root meristems per genotype were subdivided in 25 µm × 25 µm substacks (regions). N = 14 regions from three roots (secRFP–RLP4-L1ΔECD/VHA-a1–GFP), 15 regions from four roots (secRFP–RLP4ΔECD/VHA-a1–GFP) and 16 regions from four roots (all remaining combinations). See Methods for an explanation of the box plots. e,f, CLSM sections and XZ orthogonal projections of lateral root epidermal meristem cells co-expressing pUBQ10::secRFP–RLP4ΔECD and β-oestradiol-inducible A-L2 after 12 h of treatment with DMSO or 10 µM β-oestradiol (β-oest). The experiments were conducted three times independently; representative images are shown. g–n, CLSM maximum-intensity or YZ orthogonal projections of lateral root epidermal meristem cells co-expressing pUBQ10::RLP4-ECD–RFP or pUBQ10::secRFP and YFP–RAB-A5c after 30 minutes of incubation in H2O (mock) or 500 mM sorbitol (sor). The experiments were conducted three times independently; representative images are shown. Scale bars, 5 µm.
