Extended Data Fig. 3: RLP4s are edge-restricted in Arabidopsis lateral root cells.
From: A self-regulatory cell-wall-sensing module at cell edges controls plant growth
(A-H) CSLM projections of lateral root epidermal meristem cells co-expressing pRLP4s::RLP4s–RFP or pUBQ10::RLP4s–RFP with various endomembrane markers. White arrows: colocalization of RLP4s–RFP with the respective endomembrane marker, magenta arrows: compartments uniquely labelled by RLP4s–RFP. Experiments were conducted 4 times independently, representative images are shown. (I,J) MorphoGraphX projections of lateral roots meristems co-expressing pUBQ10::RLP4s–RFP (magenta) and YFP–NPSN12 (green). Experiments were conducted at least 10 times independently, representative images are shown. (K,L) CLSM XZ/YZ projections representing transverse (TM; K) and longitudinal (LM; L) midplane sections through meristematic lateral root cells co-expressing pUBQ10::RLP4-L1–RFP (magenta) and YFP–NPSN12 (green). Experiments were conducted at least 10 times independently, representative images are shown. (M-P) CLSM maximum intensity projections of lateral root meristems co-expressing pUBQ10::RLP4s–RFP (magenta), YFP–RAB-A5c (green), and Dex-inducible dominant-negative AtRPS5a»DEX»RAB-A5cN125I after 3d on DMSO (M,N) or 10 µM Dex (O,P). Arrows indicate cell plates. Experiments were conducted 2 times independently, representative images are shown. Scale bars 5 µm (A-H) or 10 µm (I-P).
