Extended Data Fig. 1: ATPase activity assay and Circular Dichroism (CD) spectroscopy.
From: Molecular basis of chromatin remodelling by DDM1 involved in plant DNA methylation
a. Principle of ATPase activity assay. b. The ATPase activity of DDM1, data are mean ± SD (n = 3 independent experiments), two-tailed Student’s t-test was applied, *** indicates P-value < 0.001. Green dots represent individual data points. The P values are: no NCP, 6.3 × 10−8; no DDM1, 1.1 × 10−7; no DDM1/NCP, 4.6 × 10−7; no DDM1/NCP/ATP, 1.8 × 10−8. c. The SDS-PAGE of purified AtDDM1-ΔN25 (ΔN) and full-length protein (FL) stained by Coomassie brilliant blue. The proteins were purified more than 3 times with similar result. d. The ATPase activity of DDM1 FL and ΔN. Data are mean ± SD (n = 3 independent experiments), two-tailed Student’s t-test was applied with P-value of 0.14 which is greater than 0.05, suggesting no statistical significance (ns) between the two datasets. Green dots represent individual data points. e. CD spectra of AtDDM1-ΔN25, full-length (FL), and the mutants at wavelength 200–240 nm. All the proteins possess similar CD patterns, suggesting a well-folded state with similar secondary structure components.
