Extended Data Fig. 4: Yeast two-hybrid assays showing that the T418D and C323A mutations abolished phyA interaction with TZP. | Nature Plants

Extended Data Fig. 4: Yeast two-hybrid assays showing that the T418D and C323A mutations abolished phyA interaction with TZP.

From: Liquid–liquid phase separation of TZP promotes PPK-mediated phosphorylation of the phytochrome A photoreceptor

Extended Data Fig. 4

a, In vivo plate assays showing that TZP interacts with phyA(WT) and phyA(D422R), but not with phyA(T418D) in yeast cells. b, Liquid culture assays showing the interactions between TZP and phyA(WT), phyA(D422R), phyA(T418D), phyA(Y242H) and phyA(C323A) in yeast cells. Yeast cultures incubated in darkness (D) were treated with 5 min of R light alone, or 5 min of R light immediately followed by 5 min of FR light, and then incubated in D for additional 3 h. β-Galactosidase activities were measured using ONPG as the substrate (the values are mean ± s.d., n = 4 independent assays). Different letters represent significant differences indicated by one-way ANOVA with Bonferroni’s multiple comparisons test (P < 0.05). The exact P values are provided in Supplementary Table 2.

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