Extended Data Fig. 5: Multiple MPKs contribute to CS integrity downstream of the SGN3 pathway (continued). | Nature Plants

Extended Data Fig. 5: Multiple MPKs contribute to CS integrity downstream of the SGN3 pathway (continued).

From: Comparisons of two receptor-MAPK pathways in a single cell-type reveal mechanisms of signalling specificity

Extended Data Fig. 5

(a) CASP1 phenotypes of T-DNA mutants mpk2, mpk16, mpk3, mpk6, mpk2 mpk16 and two independent lines of mpk2 mpk16 CRIPSR mutants. CASP1-GFP surface views at 16-17th cell are shown. (b) Immunoblot (IB) against anti-FLAG shows accumulations of the C-terminally 3xFLAG tagged MPK2a in sgn3 CASP1-GFP background. IB against MPK6 shows accumulations of MPK6a in sgn3 CASP1-GFP background. T3 seedling roots grown on DMSO or 5 µM EST were harvested. Loading controls: IBs with anti-Actin antibody or Ponceau S staining. See Supplementary Fig. 7 for uncropped original blots. This experiment was repeated three times. (c) Allelic information of two independent mpk2 mpk16 CRIPSR mutant lines. Pink arrowheads indicate positions for homozygous deletions or insertion. Amino acid numbers in brackets indicate the size of protein fragment due to early stop codon. gRNAs used to target MPK2 and MPK16 see Supplementary Table 4.

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