Extended Data Fig. 5: HPLC-HR-MS analysis of cell wall esterified non-decarboxylated diFA from 3-[2H3]-P2, 6-[2H3]-P2, [2H3]-FA treatment groups and control.
From: A sucrose ferulate cycle linchpin for feruloylation of arabinoxylans in plant commelinids
![Extended Data Fig. 5: HPLC-HR-MS analysis of cell wall esterified non-decarboxylated diFA from 3-[2H3]-P2, 6-[2H3]-P2, [2H3]-FA treatment groups and control.](http://media.springernature.com/full/springer-static/esm/art%3A10.1038%2Fs41477-024-01781-1/MediaObjects/41477_2024_1781_Fig10_ESM.jpg?as=webp)
a, EIC of non-decarboxylated di-FA (385.0918, C20H17O8, [M-H]-). a-k refers to the EIC of non-decarboxylated di-FA. b, EIC of single FA-labeled non-decarboxylated diFA ([2H3]-non-decarboxylated diFA) (388.1106, C20H14D3O8, [M-H]-). a–j: EIC from treatment groups compared to control. c, EIC of double FA-labeled non-decarboxylated diFA ([2H6]-non-decarboxylated diFA) (391.1295, C20H11D6O8, [M-H]-). a–j: EIC from treatment groups compared to control. 1: control; 2 to 5: 13.9 μM 3-[2H3]-P2, 13.9 μM 6-[2H3]-P2, 13.9 μM [2H3]-FA and 27.8 μM [2H3]-FA treatment groups. Ion chromatogram marked red is selected to show the observed mass spectra of non-decarboxylated di-FA, [2H3]-non-decarboxylated diFA, and [2H6]-non-decarboxylated diFA from control and the treatment groups (d). d, Mass spectra of non-decarboxylated diFA (Calcd. of C20H17O8 is 385.0918, [M-H]-), [2H3]-non-decarboxylated diFA (Calcd. of C20H14D3O8 is 388.1106, [M-H]-), [2H6]-non-decarboxylated diFA (Calcd. of C20H11D6O8 is 391.1295, [M-H]-) from control and 13.9 μM 3-[2H3]-P2, 13.9 μM 6-[2H3]-P2, 13.9 μM [2H3]-FA, 27.8 μM [2H3]-FA treatment groups.
