Extended Data Fig. 8: Cross-reactivity of CsLinker with green lineage Rubiscos.

a, Droplet sedimentation assays comparing the cross-reactivity of CsLinker and EPYC1 fixed at 4 μM and green Rubiscos fixed at 2 μM. In these experiments, tagged linker was also included at 5% molar ratio as completed in the accompanying microscopy experiments in Fig. 5. The amount of Rubisco and Linker pelleted in each reaction is indicated below, with the numbers colored green if droplet formation was observed in the accompanying microscopy experiments in Fig. 5. b, Confocal fluorescence microscopy and brightfield images of droplets formed with CsLinker fixed at 2 μM, with Rubiscos from the green lineage fixed at 1 μM. mEGFP-CsLinker was included at a 5% molar ratio. c, Images of droplets formed with EPYC1 fixed at 2 μM ( + 5% EPYC1-mEGFP molar ratio), with Rubiscos from the green lineage fixed at 1 μM. Scale bar in b and c is 5 μm. d, Droplet sedimentation assays comparing the cross-reactivity of CsLinker and EPYC1 fixed at 2 μM, with Rubiscos from the green lineage fixed at 1 μM. The amount of Rubisco and Linker pelleted in each reaction is indicated below, with the numbers colored green if droplet formation was observed in the accompanying microscopy experiments in b and c. Abbreviations: Cs = Chlorella sorokiniana, Cr = Chlamydomonas reinhardtii, Um = Ulva mutabilis, Ar = Adiantum raddianum (Fern), So = Spinacia oleracea, Nb = Nicotiana benthamiana, D86H = Chlamydomonas reinhardtii with D86H mutation made in RbcL.