Extended Data Fig. 6: The growth and gene expression response under cytokinin treatment in K. nitens.
From: Co-option of plant gene regulatory network in nutrient responses during terrestrialization
(a) Representative images of K. nitens grown on full Yamagami media supplied with different concentrations of BA. (b) Pixel intensity, measured as a grayscale value ranging from 0 to 255, was used as an indicator of K. nitens growth. Data are shown in mean ± sd (n = 6 colonies). (c) Relative fold changes of KnRRA1, KnRRA2, and KnRRB affected by 30 µM BA for 1 hr or 6 hrs (n = 5 biological replicates). Data are shown in mean ± sd. The K. nitens CitS (citrate synthase) gene was used as the endogenous control, and gene expression was calculated by 2-ΔCT method. ns, no significance by Student’s t-test at P value of 0.05.
