Extended Data Fig. 5: GNSR induction during bacterial colonization.

(A–C) Validation of data shown in Fig. 3. (A) Induction dynamics of GNSR genes CYP71A12 (top) and AZIL (bottom) in A. thaliana Col-0 wild-type plants during colonization by Arthrobacter Leaf137 and Rhizobium Leaf68 in mono-association over the course of nine days. Inoculation at 0 d with inoculum density of OD₆₀₀ = 0.02. Points indicate mean log₂FCs of normalized gene expression relative to axenic control plants. Error bars indicate propagated standard error. Data from one experiment with n = 1 replicates comprising 18 pooled plants per condition measured in duplicate. (B) Normalized and (C) absolute abundance of Arthrobacter Leaf137 and Rhizobium Leaf68 in planta during nine days of colonization. Bars indicate median, hinges indicate first and third quartile, whiskers indicate smallest/largest value within 1.5 × IQR (interquartile range). Data from one experiment with n = 9 plants per condition. (D) Induction of GNSR genes CYP71A12 and AZIL (y-axis) by Arthrobacter Leaf137 and Rhizobium Leaf68 two days after inoculation at indicated density (x-axis). Points indicate mean log₂FCs of normalized gene expression relative to axenic control plants. Error bars indicate propagated standard error. Data from one experiment with n = 1 replicates comprising 12 pooled plants per condition measured in duplicate. (E) Normalized bacterial abundance (y-axis) in planta two days after inoculation at indicated inoculum density (x-axis). Leaf microbiota strains (as in D) indicated above each plot. Bars indicate median, hinges indicate first and third quartile, whiskers indicate smallest/largest value within 1.5 × IQR. Data from one experiment with n = 9 plants per condition.

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